| 1 |  Arlenis Sierra Cañadilla (CUB) | Astana Women's | 1:58:30 |
| 2 | Iraida Garcia Ocasio (CUB) | | s.t. |
| 3 | Marlies Mejías Garcia (CUB) | Twenty20 - Sho-Air | s.t. |
| 4 | Claudia Barcó Arango (CUB) | | s.t. |
| 5 | Yudelmis Dominguez Masague (CUB) | | s.t. |
| 6 |  Paola Andrea Muñoz Grandon (CHI) | Swapit Agolico | s.t. |
| 7 |  Estefania Pilz (ARG) | | s.t. |
| 8 |  Lizbeth Yareli Salazar Vázquez (MEX) | Swapit Agolico | s.t. |
| 9 |  Fabiana Andrea Granizal (URU) | | s.t. |
| 10 | Mailin Sánchez Borges (CUB) | | s.t. |
| 11 | Mayra del Rocio Rocha Guerrero (MEX) | | s.t. |
| 12 | Paula Constanza Villabalon Sanchez (CHI) | | s.t. |
| 13 |  Flávia Oliveira (BRA) | Health Mate - Cyclelive | s.t. |
| 14 |  Diana Carolina Peñuela Martinez (COL) | United Health Care | s.t. |
| 15 | Laura Sofia Castillo Murillo (COL) | Pedalea - Los Cabos - JB Ropa Deportiva | s.t. |
| 16 | Julia Sanchez Parma (ARG) | | s.t. |
| 17 |  Araceli Jazmín Galeano Centurión (PAR) | | s.t. |
| 18 | Clemilda Fernandes Silva (BRA) | | s.t. |
| 19 | Ana Paula Polegatch (BRA) | Memorial - Santos - Fupes | s.t. |
| 20 |  Jennifer Mariana Cesar Salazar (VEN) | | s.t. |
| 21 | Xoana Acosta (ARG) | | s.t. |
| 22 | Scarlet Johanna Cortes Ugarte (CHI) | | s.t. |
| 23 |  Vanessa Manne (GPE) | | s.t. |
| 24 |  Marcela Rubiano Garces (CRC) | | s.t. |
| 25 | Cristina Irma Greve (ARG) | Weber - Shimano Ladies Power | s.t. |
| 26 | Jesica Bonilla Escapite (MEX) | | s.t. |
| 27 | Wilmarys del Valle Moreno Duran (VEN) | | s.t. |
| 28 | Fernanda Paola Soto Subiabre (CHI) | | s.t. |
| 29 | Lilibeth de Carmen Chacón Garcia (VEN) | S.C. Michela Fanini Record Rox | s.t. |
| 30 | Laura Gueppois (GPE) | | s.t. |
| 31 | Thayná Araujo de Lima (BRA) | Memorial - Santos - Fupes | s.t. |
| 32 | Zuralmy Coromoto Rivas Molina (VEN) | | s.t. |
| 33 | Danielys del Valle García Buitriag (VEN) | | s.t. |
| 34 | Milagro Consuelo Mena Solano (CRC) | | s.t. |
| 35 | Silvia Maria Rodas Maldonado (PAR) | | s.t. |
| 36 | Gigliolla Monichi (CHI) | | s.t. |
| 37 |  Lisa Groothuesheidkamp (CUR) | | s.t. |
| 38 | Serika Mitchell Guluma Ortiz (COL) | | s.t. |
| 39 |  Juana Isidra Fernandez Veras (DOM) | | s.t. |
| 40 | Adriana Maria Rojas Cubero (CRC) | | s.t. |
| 41 | Carla Raquel Moncher (URU) | | s.t. |
| 42 | Aranza Valentina Villalon Sanchez (CHI) | | s.t. |
| 43 | Jeny Lorena Colmenares Colmenares (COL) | | s.t. |
| 44 |  Miryan Maritza Nuñez Padilla (ECU) | | s.t. |
| 45 | Luisa Fernanda Naranjo Henao (COL) | Pedalea - Los Cabos - JB Ropa Deportiva | s.t. |
| 46 | Maria José Vargas Barrientos (CRC) | Swapit Agolico | s.t. |
| 47 | Ana Teresa Casas Bonilla (MEX) | Swapit Agolico | s.t. |
| 48 |  Jazmín Gabriela Soto Lopez (GUA) | | s.t. |
| 49 | Talita Da Luz De Oliveira (BRA) | | s.t. |
| 50 | Marcia Fernandes Silva (BRA) | | s.t. |
| 51 | Maribel Roxana Aguirre Mangue (ARG) | Weber - Shimano Ladies Power | s.t. |
| 52 | Brenda Andrea Santoyo Perez (MEX) | | s.t. |
| 53 | Cintia Eugenia Lee Lopez (GUA) | | s.t. |
| 54 | Florida Isabel De León Gonzalez (GUA) | | s.t. |
| 55 | Andrea Ramírez Fregoso (MEX) | Swapit Agolico | s.t. |
| 56 | Paula Cristina Herrera Hernandez (CRC) | | s.t. |
| 57 |  Angela Francisca Ramírez Alva (PER) | | s.t. |
| 58 | Natalia Navarro Cerdas (CRC) | | s.t. |
| 59 | Angie Marielle Paulet Bustamante (PER) | | s.t. |
| 60 | Kerla Excelsa De Jesus Rodriguez (DOM) | | 0:12 |
| 61 | Ana Cristina Sanabria Sanchez (COL) | Servetto - Stradalli Cycle - Alurecycling | 0:12 |
| 62 | Barbara Fiorela Malaspina (ARG) | | 0:12 |
| 63 | Angie Sabrina Gonzalez Garcia (VEN) | | 1:31 |
Abigail Sarabia Ricaldez (BOL)